Characterization of the Yeast Ionome: A Genome-Wide Analysis of Nutrient Mineral and Trace Element Homeostasis in Saccharomyces Cerevisiae

Authors

David J. Eide, University of Wisconsin-Madison
Suzanne Clark, University of Wisconsin-Madison
T . Murlidharan Nair, University of California - San Diego
Mathias Gehl, University of Nevada, Reno
Michael Gribskov, University of California - San Diego
Mary Lou Guerinot, Dartmouth CollegeFollow
Jeffrey Harper, University of Nevada, Reno

Document Type

Article

Publication Date

8-30-2005

Publication Title

Genome Biology

Department

Department of Biological Sciences

Abstract

Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur, and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'.

DOI

10.1186/gb-2005-6-9-r77

Dartmouth Digital Commons Citation

Eide, David J.; Clark, Suzanne; Nair, T . Murlidharan; Gehl, Mathias; Gribskov, Michael; Guerinot, Mary Lou; and Harper, Jeffrey, "Characterization of the Yeast Ionome: A Genome-Wide Analysis of Nutrient Mineral and Trace Element Homeostasis in Saccharomyces Cerevisiae" (2005). Dartmouth Scholarship. 892.
https://digitalcommons.dartmouth.edu/facoa/892