Document Type
Article
Publication Date
10-27-2016
Publication Title
Genome Medicine
Department
Geisel School of Medicine
Abstract
Homologous recombination (HR) is the primary pathway for repairing double-strand DNA breaks implicating in the development of cancer. RNAi-based knockdowns of BRCA1 and RAD51 in this pathway have been performed to investigate the resulting transcriptomic profiles. Here we propose a computational framework to utilize these profiles to calculate a score, named RNA-Interference derived Proliferation Score (RIPS), which reflects cell proliferation ability in individual breast tumors. RIPS is predictive of breast cancer classes, prognosis, genome instability, and neoadjuvant chemosensitivity. This framework directly translates the readout of knockdown experiments into potential clinical applications and generates a robust biomarker in breast cancer.
DOI
10.1186/s13073-016-0363-3
Dartmouth Digital Commons Citation
Wang, Yue; Mark, Kenneth . M. K.; Ung, Matthew H.; Kettenbach, Arminja; Miller, Todd; Xu, Wei; Cheng, Wenqing Cheng; Xia, Tian; and Cheng, Chao, "Application of RNAi-Induced Gene Expression Profiles for Prognostic Prediction in Breast Cancer" (2016). Dartmouth Scholarship. 894.
https://digitalcommons.dartmouth.edu/facoa/894
