Document Type

Article

Publication Date

12-2000

Publication Title

Infection and Immunity

Department

Geisel School of Medicine

Abstract

We have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific host membrane receptor for parasite attachment that is upregulated during the mid-S phase of the host cell cycle.

DOI

10.1128/IAI.68.12.7198-7201.2000

Original Citation

Dutta C, Grimwood J, Kasper LH. Attachment of Toxoplasma gondii to a specific membrane fraction of CHO cells. Infect Immun. 2000 Dec;68(12):7198-201. doi: 10.1128/iai.68.12.7198-7201.2000. PMID: 11083856; PMCID: PMC97841.

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