Document Type
Article
Publication Date
12-2000
Publication Title
Infection and Immunity
Department
Geisel School of Medicine
Abstract
We have observed previously that attachment of Toxoplasma gondii to synchronized host cells is considerably increased at the mid-S phase (4 h postrelease). Synchronized CHO host cells at the mid-S phase were fractionated by molecular weight, and the antigens were used to produce a panel of polyclonal mouse antisera. The polyclonal antisera raised against fraction 4 with molecular mass ranging approximately from 18 to 40 kDa significantly reduced attachment to mid-S-phase host cells. Immunofluorescence assays demonstrated strong reactivity to mid-S-phase host cells and identified a number of potential receptors on Western blots. These data indicate that there is a specific host membrane receptor for parasite attachment that is upregulated during the mid-S phase of the host cell cycle.
DOI
10.1128/IAI.68.12.7198-7201.2000
Original Citation
Dutta C, Grimwood J, Kasper LH. Attachment of Toxoplasma gondii to a specific membrane fraction of CHO cells. Infect Immun. 2000 Dec;68(12):7198-201. doi: 10.1128/iai.68.12.7198-7201.2000. PMID: 11083856; PMCID: PMC97841.
Dartmouth Digital Commons Citation
Dutta, Chaitali; Grimwood, Jane; and Kasper, Lloyd H., "Attachment of Toxoplasma gondii to a Specific Membrane Fraction of CHO Cells" (2000). Dartmouth Scholarship. 979.
https://digitalcommons.dartmouth.edu/facoa/979
