Document Type

Article

Publication Date

4-15-2011

Publication Title

Biomedical Optics Express

Department

Thayer School of Engineering

Additional Department

Geisel School of Medicine

Abstract

A two-channel optical system has been developed to provide spatially resolved simultaneous imaging of singlet molecular oxygen (1O2) phosphorescence and photosensitizer (PS) fluorescence produced by the photodynamic process. The current imaging system uses a spectral discrimination method to differentiate the weak 1O2 phosphorescence that peaks near 1.27 μ m from PS fluorescence that also occurs in this spectral region. The detection limit of 1O2 emission was determined at a concentration of 500 nM benzoporphyrin derivative monoacid (BPD) in tissue-like phantoms, and these signals observed were proportional to the PS fluorescence. Preliminary in vivo images with tumor laden mice indicate that it is possible to obtain simultaneous images of 1O2 and PS tissue distribution.

DOI

10.1364/BOE.2.001233

Original Citation

Lee S, Isabelle ME, Gabally-Kinney KL, Pogue BW, Davis SJ. Dual-channel imaging system for singlet oxygen and photosensitizer for PDT. Biomed Opt Express. 2011 Apr 15;2(5):1233-42. doi: 10.1364/BOE.2.001233. PMID: 21559134; PMCID: PMC3087579.

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