Protein-Protein Fusion Catalyzed by Sortase A

Authors

David A. Levary, Harvard University
Ranganath Parthasarathy, University of Tennessee, Knoxville
Eric T. Boder, University of Tennessee, Knoxville
Margaret E. Ackerman, Dartmouth CollegeFollow

Document Type

Article

Publication Date

4-6-2011

Publication Title

PloS One

Department

Thayer School of Engineering

Abstract

Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality — demonstrating the robust and facile nature of this reaction.

DOI

10.1371/journal.pone.0018342

Original Citation

Levary DA, Parthasarathy R, Boder ET, Ackerman ME. Protein-protein fusion catalyzed by sortase A. PLoS One. 2011 Apr 6;6(4):e18342. doi: 10.1371/journal.pone.0018342. PMID: 21494692; PMCID: PMC3071835.

Dartmouth Digital Commons Citation

Levary, David A.; Parthasarathy, Ranganath; Boder, Eric T.; and Ackerman, Margaret E., "Protein-Protein Fusion Catalyzed by Sortase A" (2011). Dartmouth Scholarship. 3130.
https://digitalcommons.dartmouth.edu/facoa/3130